BMP-2 Human Recombinant Protein

Product Description Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily of proteins. It plays a crucial role in the development and maintenance of bone and cartilage. BMP-2 is involved in various cellular processes, including cell differentiation, proliferation, and apoptosis. Our products are produced using a proprietary plant based expressed system. Animal Component Free (ACF). No endotoxins, prions or virons to risk microbial contamination Highly flexible platform used to produce multiple proteins with minimal changes to method Our proteins generally have 10% to 20% higher activity Current Lead Time 1-2 Working Days  Product Specifications Species Human* Expression System Nicotiana Benthamiana Purity >95% by SDS-PAGE Activity 0.3-3.75 ng/mL EC50 Activity Assay Induced HUVEC proliferation assay dataCell proliferation is measured by RezasurinrhVEGF165 MNPHARM, ED50 = 4.557 ng/mLUsing 2 mg/mL concentration measurement Animal Component Free (ACF) Yes Molecular Weight 25 kDa Structure Disulfide-linked homodimer Endotoxin Concentration <1 EU/µg Purification Sequential Chromatography Form Frozen Formulation Supplied as a 0.22 µm filtered solution, 50 mM MES, 10% glycerol, pH 5 *For Research Use Only Storage Instructions Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles." Sample Prep Instructions  Thaw the vial on ice, spin briefly Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered). Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C) We recommend sterile filtering after dilution in media or the final working solution Bioinformatics Alternative Names:  UniProt ID: (Human) P12643 Entrez Gene ID: (Human) 55589 Documents & Downloads