T7 RNA Polymerase ELISA Kit _ 36705ES

Product description T7 RNA polymerase that synthesizes RNA complementary to reverse single-stranded DNA downstream of the promoter using double-stranded DNA containing the T7 promoter sequence (5'-TAATACGACT CACTATAG*-3') as a template and NTP as a substrate. This kit uses the principle of double-antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residual T7 RNA polymerase in samples. First, add the T7 RNA polymerase standard and test sample to the Anti-T7 RNA Polymerase coated microtiter strips (36705-A), then add the diluted biotin labeled Anti-T7 RNA polymerase antibody (36705-C), and finally add Streptavidin-HRP (36705-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. Subsequently, add TMB substrate (36705-H) into the complex to observe color reaction after washing the complex. TMB is converted into blue under the catalysis of HRP enzyme and finally converted into yellow in the presence of acid, and the shade of color is positive