Volvocacean-3N Medium

Volvocacean-3N Medium R.C. Starr's modification of Volvocacean medium (E.G. Pringsheim, pers. Comm.), i.e., the amount of (NH4)2HPO4 is increased 3-fold, for axenic cultures of Eudorina (1204, 1205 and 1206) and other colonial volvocales. Directions For 1 L Total To approximately 900 mL of dH2O, add the following components in the order listed except Euglena Medium while stirring continuously. Adjust the pH to 6.0 Bring total volume to 1 L with dH2O. Discard 200 mL of this medium. Transfer 200 mL of the previously prepared Euglena medium into the flask. For agar add 10.0 grams of agar into the flask; do not mix. Autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 KNO3(Baker 3190) 1 mL/L 10 g/100 mL dH2O 0.98 mM 2 MgSO4•7H2O(Sigma 230391) 1 mL/L 2 g/100 mL dH2O 0.081 mM 3 (NH4)2HPO4(Fisher A686) 3 mL/L 2 g/100 mL dH2O 0.453 mM 4 CaSO4•2H2O(Mallinckroft 4300) 30 mL/L 0.2 g/100 mL dH2O 0.35 mM 5