Bristol Medium

Bristol Medium H.C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium and as bristol's solution, an essential component of other media--see Bold 1NV, Bold 3N, Bristol-NaCl, LDM, Proteose, Soil extract, and Trebouxia. Directions For 1 L Total To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. *For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/400mL dH2O 2.94 mM 2 CaCl2•2H2O (Sigma C-3881) 10 mL/L 1 g/400mL dH2O 0.17 mM 3 MgSO4•7H2O (Sigma 230391) 10 mL/L 3 g/400mL dH2O 0.3 mM 4 K2HPO4 (Sigma P 3786) 10 mL/L 3 g/400mL dH2O 0.43 mM 5 KH2PO4 (Sigma P 0662) 10 mL/L 7 g/400mL dH2O 1.29 mM 6 NaCl (Fisher S271-500) 10 mL/L 1 g/400mL dH2O 0.43 mM