J Medium

J Medium Modification of Parker's J (or, Jansen) medium for Microcystis. Directions For 1 L Total pH 8.3 - 8.5 To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. For 1.25% agar medium add 12.5 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Macro Component 1 for J Medium 10 mL/L 2 Macro Component 2 for J medium 10 mL/L 3 CaCl2•2H2O(Sigma C-3881) 1 mL/L 0.29 g/100 mL dH20 0.02 mM 4 G9 Trace Metals for J medium 1 mL/L