A+ Medium

A+ Medium Directions For 1 L Total Liquid media: To approximately 900 mL of dH2O add the first 8 components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. Cover and autoclave medium. Add filter sterilized A+ Trace Components. Allow to cool then store at refrigerator temperature. Agar media: To approximately 390 mL of dH2O add the first 8 components in the order specified while stirring continuously. Bring total volume to 500 mL with dH2O. In a separate container add 15 g of agar to 500 mL of dH2O (Final 1.5% w/v). Cover and autoclave both solutions. In a water bath allow both solutions to cool to 45-50 °C. Add sterile Sodium Thiosulfate to agar solution and mix well. Add filter sterilized A+ Trace Components. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly. Allow to cool then store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 NaCl(Fisher S271-5