
Rat Vascular Endothelial Growth Factor Receptor 2 ELISA Kit
Rat Vascular Endothelial Growth Factor Receptor 2 ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Vascular Endothelial Growth Factor Receptor 2 in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . VEGFR2 present in samples reacts with an anti-Rat VEGFR2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-VEGFR2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active VEGFR2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat VEGFR2. Detection Target: Rat Vascular E