Human Vascular Endothelial Growth Factor 165 ELISA Kit

Human Vascular Endothelial Growth Factor 165 ELISA Kit from Innovative Research is intended for the quantitative determination of Human Vascular Endothelial Growth Factor 165 in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . VEGF165 present in samples reacts with an anti-Human VEGF165 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-VEGF165 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active VEGF165 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human V