BCIP/NBT 50X

5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on Western, Northern, Southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive. SpecificationsOffered as 10 ml: Molecular Weight: NBT: MW = 817.7, BCIP toluidine salt: MW = 433.6Molecular Formula: NBT: ·C40H30Cl2N10O6, ·BCIP: ·C8H6NO4BrClP ·x ·C7H9NForm: LiquidPhysical Appearance: Yellow to light brown liquidConcentration: 50X Usage: Preparation of staining solution: •Add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride. (do not include Magnesium Chloride in the detection buffer, this