Ribonuclease A (RNase A) from bovine pancreas

  Description Ribonuclease A (RNase A) is a single-stranded polypeptide containing 4 disulfide bonds with a molecular weight of about 13.7 kDa. RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Specifically, the cleavage recognizes the phosphodiester bond formed by the 5'-ribose of a nucleotide and the phosphate group on the 3'-ribose of the adjacent pyrimidine nucleotide, so that the 2', 3' - Cyclic phosphates are hydrolyzed to the corresponding 3'-nucleoside phosphates (eg, pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-PG).RNase A is the most active in cleaving single-stranded RNA .Recommended working concentration is 1-100 μ G/mL, compatible with various reaction systems. Low salt concentration (0-100 mM NaCl) can be used to cut single-stranded RNA, double-stranded RNA, and RNA chains formed by RNA-DNA hybridization. However, at high salt concentration (≥ 0.3 M), RNase A only specifically cleaves single-stranded RNA