RNase A solution (10 mg/ml)

Background RNase A is commonly used in the processes of plasmid and genomic DNA purification and protein extraction, in addition to RNA sequence analysis and RNase protection assays. Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNase A is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNase A prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides. RNase A acts on single stranded and double stranded RNA. RNase A Solution The recombinant Ribonuclease A (RNase A) enzyme is produced from yeast cells with a cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.CAS No.: 9001-99-4.E.C.: 3.1.27.5.Molecular Weight: 13.7 kDa.pI: 9.6.pH range: 6-10; high activity in the pH range of 7.6-8.0.Unit Definition: One unit of the RNase A enzyme causes an increase of absorbance by 1.0 unit at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0. Fifty units are ap