Recombinant G-CSF, Rat - BK0062

Recombinant G-CSF, Rat Catalogue Numbers: BK0062-10, BK0062-50 Sizes: 10μg, 50μg Source: Escherichia coli Molecular Weight: 21.4 kDa, observed by non-reducing SDS-PAGE. Purity: > 95% by SDS-PAGE analysis. Biological Activity: ED50 < 0.1 ng/mL, measured by a cell proliferation assay using NSF-60 cells, corresponding to a specific activity of > 1 × 10ˆ7 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 20mM Citric Acid AA Sequence: MIPLLt VSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPt VQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI Endotoxin: < 0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O at 100 μg/mL. Storage: Lyophilized recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrG-CSF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description: Granulocyte Colony-Stimulating Factor (G-CSF) is a hematopoietic cytokine belonging to the four-helix bundle cytokine superfamily. G-CSF is produced by monocytes, macrophages, fibroblasts, and endothelial cells. Its expression is highly regulated and induced by a variety of agents, including Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interferon γ (IFN-γ), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). G-CSF binds to the CSF-specific high affinity receptors expressed on neutrophilic granulocyte lineage. In vivo G-CSF regulates the production of neutrophilic granulocytes, a critical part of host defense systems, and helps the maturation of leukemic cell lines. G-CSF is widely employed clinically because of its fairly innocuous safety profile.Recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) produced in E.coli is a single non-glycosylated polypeptide chain containing of 194 amino acids. A fully biologically active molecule, rrG-CSF has a molecular mass of 21.4 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.