Recombinant G-CSF, Human - BK0061

Recombinant G-CSF, Human Catalogue Numbers: BK0061-10, BK0061-50 Sizes: 10μg, 50μg Source: Escherichia coli Molecular Weight: 18.8kDa, observed by reducing SDS-PAGE. Purity: > 95% by SDS-PAGE and HPLC analyses. Biological Activity: ED50 <0.1ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of >1.0× 10ˆ7 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 25mM Tris, pH8.0. AA Sequence: MTPLGPASS LPQSFLLKCL EQVRKIQGDG AALQEKLCAT YKLCHPEELV LLGHSLGIPW APLSSCPSQA LQLAGCLSQL HSGLFLYQGL LQALEGISPE LGPTLDTLQL DVADFATTIW QQMEELGMAP ALQPTQGAMP AFASAFQRRA GGVLVASHLQ SFLEVSYRVL RHLAQP Endotoxin: <0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O at 100 μg/ml. Storage: Lyophilized recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description: Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.