Trebouxia Medium

Trebouxia Medium Modification of Ahmadjian's medium for the phycobionts of lichens. Suitable for most axenic cultures of Trebouxia, Pseudotrebouxia, Trentepohlia and Cephaleuros. Directions For 1 L Total Prepare 1 L of Bristol Medium. Transfer approximately 825 mL to a 2-L Erlenmeyer flask. Save the remaining Solution. To approximately 825 mL of Bristol Medium, add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with the Bristol Medium saved from step 1. For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Bristol Medium 825 mL/L 2 Soilwater: GR+ Medium 140 mL/L 3 Proteose Peptone(BD 211684) 10 g/L 4 Glucose(BACTO-dextrose or Sigma D 9634) 20 g/L 0.11 M