Soilwater: VT Medium

Soilwater: VT Medium Soil having little or no organic content is substituted for garden-type soil; lacks supplements; for some strains of Closterium, freshwater dinoflagellates and others. Directions For 200 mL Total Combine components. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature. Refrigerate 24 hours or more and bring to room temperature before use. # Component Amount Stock Solution Concentration Final Concentration 1 Vermont Soil 1 tsp 2 dH2O 200 mL