Sambucus nigra Lectin (SNA/EBL I+II) - Pure

Affinity purified Sambucus nigra lectin (EBL I+II, also known as SNA) is is composed of two ricin-related lectins. EBL I+II is a tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000D and two of about 38,000D with a recognition of the Neu5Ac(a2-6)Gal or GalNAc linkage in glycoconjugates. EBL I+II is not blood group specific but it does agglutinate type A erythrocytes more strongly than either B or O cells. Trypsin treated erythrocytes react better than untreated cells. Neuraminidase treated erythrocytes react with this lectin due to the presence of terminal galactose resides. SNA has a broad specificity for both ?- and ?-linked galactose, as well as unrelated sugars such as fucose. It has been determined that the lectin does not bind to glycoproteins or glycolipids containing only terminal ?(2,3)-linked sialic acid residues.EBL I is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. EBL II consists only of