5’-Nucleotidase (5'-NT) Activity Assay Kit

5’-Nucleotidase (5'-NT) Activity Assay Kit SKU # E-BC-K196-M Detection Instrument Microplate reader (550 nm) Detection method Colorimetric method Product Details Properties Synonyms 5’-NT Sample Type Serum, plasma, animal tissue Sensitivity 28.0 U/L Detection Range 28.0-581 U/L Detection Method Colorimetric method Assay type Quantitative Assay time 30 min Precision Average inter-assay CV: 6% | Average intra-assay CV: 3.300% Other instruments required Pipettor, Water bath, Centrifuge Other reagents required Normal saline (0.9% NaCl) Storage 2-8℃ Valid period 12 months Detection Principle 5'-Nucleotide enzyme (5'-NT, EC 3.1.3.5), full name is 5'-ribonucleotide phosphohydrolase, is a special phosphohydrolase that specifically hydrolyzes 5'-phosphoric acid attached to pentose in 5'-nucleotide. This enzyme is widely distributed in the cell membrane of various tissues of human and animal. Only the 5'-NT released by the tissue cells of the hepatobiliary