Rat Wolfram Syndrome Protein 1 ELISA Kit

Rat Wolfram Syndrome Protein 1 Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Wolfram Syndrome Protein 1 in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . WFS1 present in samples reacts with an anti-Rat WFS1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-WFS1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active WFS1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat WFS1. Detection Target: Rat Wolfram Syndrome Protein 1 Reactivity: Rat Range: 0.312