Rat Interferon Gamma Induced Protein 10kDa ELISA Kit

Rat Interferon Gamma Induced Protein 10kDa ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Interferon Gamma Induced Protein 10kDa in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . IP10 present in samples reacts with an anti-Rat IP10 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-IP10 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active IP10 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat IP10. Detection Targe