Porcine Transforming Growth Factor Beta 1 ELISA Kit

Porcine Transforming Growth Factor Beta 1 ELISA Kit from Innovative Research is intended for the quantitative determination of Porcine Transforming Growth Factor Beta 1 in biofluid samples, such as serum, platelet-poor plasma, tissue homogenates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . TGFb1 present in samples reacts with an anti-Porcine TGFb1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-TGFb1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active TGFb1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Porcine TGFb1. Dete