Mouse X-Box Binding Protein 1 ELISA Kit

Mouse X-Box Binding Protein 1 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse X-Box Binding Protein 1 in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . XBP1 present in samples reacts with an anti-Mouse XBP1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-XBP1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active XBP1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse XBP1. Detection Target: Mouse X-Box Binding Protein 1 Reactivity: Mouse Range: 0.156-10ng/mL Sensit