Human C1 Inhibitor ELISA Kit

This Human C1 Inhibitor ELISA Kit from Innovative Research is intended for Quantitative Detection of human Serpin G1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. This human Serpin G1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin G1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: N23-A500) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin G1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The de