Bauhinia purpurea Lectin (BPL/BPA) - Biotinylated

Affinity purified Bauhinia purpurea agglutinin or lectin (BPA/BPL) (SKU: 21510028) is a tetrameric lectin with a molecular weight of 120 kDa. Binding is generally highest for glycoconjugates containing galactosyl (ß-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind to BPL. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. Although the binding specificity of BPA is similar to that of peanut agglutinin, the tissue staining patterns of these two lectins are distinct. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the