Puromycin Solution, 10mg/ml , Cell Cultere Tested

Puromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 μg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 μg/ml. Puromycin resistant stable mammalian cell lines can be generated in less than one week. Store at -20℃ Concentraion 10mg/ml. This product is soluble in water at 10 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C Mode of Action Puromycin inhibits protein synthesis