CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Super Bright™ 702, eBioscience™

PRODUCT DETAILSHost: RatIsotype: IgG2b, kappaClonality: MonoclonalClone: DTA-1Format: Super Bright™ 702Reactivity: MouseApplication: Flow CytometryTested Dilution: 0.3 µg/testConcentration: 0.2 mg/mLStorage: 4° C, store in dark, DO NOT FREEZE!Formulation: PBS, pH 7.2, containing 0.09% sodium azidePurification: Affinity chromatographyData Sheet: TDS Specific InformationDescription: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.