DAB Liquid Concentrate 50X

DAB (3-3′ diaminobenzidine tetrachloride) is suitable for HRP-based immunoblotting and immunohistochemical assays. Oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium. Specifications: Synonym: 3,3' Diaminobenzidine TetrahydrochlorideCAS Number: 7411-49-6Molecular Weight: 360.1Molecular Formula: C12H14N4 ·4HClForm: LiquidPhysical Appearance: dark brown solutionSolubility: Insoluble in most organic solventsConcentration: 50X concentrate   Storage: Store at 4° C. Discard if a precipitate forms or if reagent is purple (the purple material is a DAB oxidation product that binds avidly to heme-containing proteins).