Biotinylated Human CD3E/CD3 epsilon 1-27 Protein, Active

Product Overview Description Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains Asp23-Thr48. Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC Accession P07766 Target Symbol CD3E/CD3 epsilon 1-27 Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 Species Human Expression System HEK293 Tag C-hFc-Avi Expression Range Asp23-Thr48 Mol. Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27, hFc Tag with the EC50