CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

Description This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. Note: G* is the first base of the RNA transcript. Feature dsRNA level lower to about 1/100000 Comptiable with Trilink CleanCap AG High yields comparable to WT  Lower Cap input Animal origin-free (AOF) Please find information on the development of this enzyme. Components Components No. Name 10628ES10 10628ES60 10628ES72 10628ES86     (10 KU) (100 KU) (250 KU) (2,500 KU) 10628 CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)