Zymolyase, 20T (20 KU/g), from Arthrobacter luteus

  Zymolyase produced by Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It hydrolyzes linear glucose polymers at beta-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Appearance: Light brown, lyophilized powder Storage: 4 °C Activity: 22,000 U/g Essential enzyme: Beta-1,3-glucan laminaripentaohydrolase Other enzyme components: Protease, Mannanase, and trace amount of Amylase, Xylanase, Phosphatase Stable pH: 5-10 Optimum working pH and temperature condition: For lysis of viable yeast cells: pH 7.5, 35°C. For hydrolysis of yeast glucan: pH 6.5, 45°C. Heat stability: At 30°C, 70% of lytic activity is lost after storage for 3 months At 60°C, lytic activity is lost on incubation for 5 minutes Activator: Cystein, 2-mercaptoethanol, Dithiothreitol etc.   *Unit definition: One unit of lytic activity is defined as that amount which indicate