Recombinant MCP-1/CCL2, Human - BK0125

Recombinant MCP-1/CCL2, Human Catalogue Numbers: BK0125-5, BK0125-25 Sizes: 5μg, 25μg Source: Escherichia coli Molecular Weight: 8.7 kDa, observed by reducing SDS-PAGE. Purity: > 95% as analyzed by SDS-PAGE. Biological Activity: The EC50 value of human MCP-1/CCL2on Caˆ2+ mobilization assay in CHO-K1/Gα15/hCCR2 cells (human Gα15 and human CCR2 stably expressed in CHO-K1 cells) is less than 1μg /ml. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against PBS. AA Sequence: QPD AINAPVTCCY NFTNRKISVQ RLASYRRITS SKCPKEAVIF KTIVAKEICA DPKQKWVQDS MDHLDKQTQT PKT Endotoxin: < 0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml. Storage: Lyophilized recombinant Human MCP-1/CCL2 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human MCP-1/CCL2 should be stable up to 1 week at 4°C or up to 3 months at -20°C. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description: Chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemotactic protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein. Human CCL2 shares 78-79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region.Recombinant Human MCP-1/CCL2 produced in E.coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-1/CCL2 has a molecular mass of 8.7 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.