Recombinant Human Interleukin-1 beta (rHu IL-1β) - PR1055

Recombinant Human Interleukin-1 beta (rHu IL-1β) Catalogue Numbers: PR1055-2, PR1055-10 Sizes: 2µg, 10µg Source: Escherichia coli Molecular Weight: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. Purity: >97% by SDS-PAGE and HPLC analyses. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less than 1.0 pg/ml, corresponding to a Specific Activity of ＞1.0 x 109 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.5. AA Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS Endotoxin: Less than 1EU/mg of rHuIL-1β as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China Description: IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.