Rat IGF-2 (Insulin Like Growth Factor 2) CLIA Kit - E-CL-R0367

Rat IGF-2 (Insulin Like Growth Factor 2) CLIA Kit Sizes: 24T, 48T, 96T Catalogue Numbers: E-CL-R0367-24, E-CL-R0367-48, E-CL-R0367-96 Citations, Manuals and MSDS Available upon request. Abbreviation: IGF-2 UNIProt ID: P01346 Target Synonyms: IGF2; IGF-II; Research Areas: Cancer, Developmental biology, Signal transduction Target Species: Rat Application: CLIA Usage: This ELISA kit applies to the in vitro quantitative determination of Rat IGF-2 concentrations in serum, plasma and other biological fluids. Tested Sample Types: Serum, Plasma and other biological fluids Type: Sandwich Assay Time: 3.5h Detection Type: Chemiluminescence Sensitivity: 7.5 pg/mL Detection Range: 12.5-800 pg/mL Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF-2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IGF-2. You can calculate the concentration of Rat IGF-2 in the samples by comparing the RLU value of the samples to the standard curve. Cross Reactivity: This kit recognizes Rat IGF-2 in samples. No significant cross-reactivity or interference between Rat IGF-2 and analogues was observed Recovery Rate: 80%-120% Precision: Both intra-CV and inter-CV are < 10%. Storage: 2-8℃/-20℃, 6 months Shipping: ice bag