Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit - E-CL-R0312

Rat GDF3 (Growth Differentiation Factor 3) CLIA Kit Sizes: 24T, 48T, 96T Catalogue Numbers: E-CL-R0312-24, E-CL-R0312-48, E-CL-R0312-96 Citations, Manuals and MSDS Available upon request. Abbreviation: GDF3 UNIProt ID: A2SY89 Research Areas: Signal transduction, Stem cells Target Species: Rat Application: CLIA Usage: This ELISA kit applies to the in vitro quantitative determination of Rat GDF3 concentrations in Serum, plasma and other biological fluids. Tested Sample Types: Serum, Plasma and other biological fluids Type: Sandwich Assay Time: 3.5h Detection Type: Chemiluminescence Sensitivity: 9.38 pg/mL Detection Range: 15.63-1000 pg/mL Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GDF3. You can calculate the concentration of Rat GDF3 in the samples by comparing the RLU value of the samples to the standard curve. Cross Reactivity: This kit recognizes Rat GDF3 in samples. No significant cross-reactivity or interference between Rat GDF3 and analogues was observed Recovery Rate: 80%-120% Precision: Both intra-CV and inter-CV are < 10%. Storage: 2-8℃/-20℃, 6 months Shipping: ice bag